How does sirna

Evolution of microRNA diversity and regulation in animals. Efficient in vivo delivery of siRNA to the liver by conjugation of alpha tocopherol. Role for a bidentate ribonuclease in the initiation step of RNA interference. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells.

Expression profiling reveals off-target gene regulation by RNAi. Chemical modification of siRNAs to improve serum stability without loss of efficacy. Biochem Biophys Res Commun. Argonaute2 is the catalytic engine of mammalian RNAi. Tomari Y, Zamore PD. Perspective: machines for RNAi.

Genes Dev. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Meister G, Tuschl T. Mechanisms of gene silencing by doublestranded RNA. Schaffert D, Wagner E. Gene therapy progress and prospects: synthetic polymerbased systems. Kumar P, et al. Cationic lipids and polymers mediated vectors for delivery of siRNA. Control Release. Transvascular delivery of small interfering RNA to the central nervous system.

Gene delivery by cationic lipid vectors: overcoming cellular barriers. Supramolecular assemblies in functional siRNA delivery: where do we stand? Verma V, Sangave P. Non-viral vectors for gene-based therapy. Nat Rev Genet. Li W, Szoka FC.

Pharm Res. Novel targeted liposomes deliver sirna to hepatocellular carcinoma cells in vitro. Chem Biol Drug Des. Efficient siRNA delivery with non-viral polymeric vehicles. Lowmolecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine. Control Rel. A pH-sensitive cationic lipid facilitates the delivery of liposomal siRNA and gene silencing activity in vitro and in vivo.

J Control Release. Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform. Nanomedicine Lond ; 8 — Therapeutic targeting in the silent era: advances in non-viral siRNA delivery. Zhang XLi, Huang L. Non-viral nanocarriers for siRNA delivery in breast cancer. Liposome-siRNApeptide complexes cross the blood-brain barrier and significantly decrease PrP on neuronal cells and PrP in infected cell cultures. PLoS One. Drug Target. Akhtar S, Benter IF.

Clin J. Expert Opin. Drug Delivery. Polymer-based siRNA delivery: perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery.

Kortylewski M, et al. Optimized galenics improve in vitro gene transfer with cationic molecules up to fold. Soutschek J, et al. Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs.

Rozema DB, et al. Natl Acad. Hunter AC. Molecular hurdles in polyfectin design and mechanistic background to polycation induced cytotoxicity. Drug Deliv. Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion. A pH-sensitive polymer that enhances cationic lipid-mediated gene transfer. Bioconjug Chem. Rajeev KG, et al. Hepatocyte-specific delivery of siRNAs conjugated to novel non-nucleosidic trivalent N-acetylgalactosamine elicits robust gene silencing in vivo.

Copolymers of ethylene imine and N- 2-hydroxyethyl -ethyleneimine as tools to study effects of polymer structure on physicochemical and biological properties of DNA complexes. Biessen EA, et al. Synthesis of cluster galactosides with high affinity for the hepatic asialoglycoprotein receptor. Wong SC, et al.

Co-injection of a targeted, reversibly masked endosomolytic polymer dramatically improves the efficacy of cholesterolconjugated small interfering RNAs in vivo. Nucleic Acid Ther. A role for peptides in overcoming endosomal entrapment in siRNA delivery — a focus on melittin. Biotechnol Adv. Metabolic stability of glutaraldehyde cross-linked peptide DNA condensates.

Multifunctional siRNA delivery system: polyelectrolyte complex micelles of six-arm PEG conjugate of siRNA and cell penetrating peptide with crosslinked fusogenic peptide. Fusogenic peptides enhance endosomal escape improving siRNAinduced silencing of oncogenes. Song E, et al. Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors. This process serves important roles in regulating protein synthesis, whether in general or of specific proteins, by acting as a layer of control separate and downstream from the various genes that regulate transcription itself.

In particular, RNA interference prevents mRNAs from outlasting their need, by disposing of them before they might otherwise naturally degrade. RNA interference shows fascinating promise as a tool in molecular biology. These RNAs then inhibit protein synthesis in a less complicated and invasive way than altering the genetic code. As a new area of protein synthesis regulation to explore, RNA interference is a fertile area of inquiry in many sub-fields of medicine.

It may become an important weapon against increasingly antibiotic-resistant bacterial infections, inhibiting bacteria in an entirely different way than antibiotics do. It is another possibility to explore in the fight against cancer, disabling protein synthesis inside cancer cells until they die or at least cease reproducing.

III, Parker, R. Lund, E. Nuclear export of microRNA precursors. Science , 95— Ma, J. Maida, Y. Biol Chem , — Meister, G. Mechanisms of gene silencing by double-stranded RNA.

Cell 15, — Identification of novel argonaute-associated proteins. Mello, C. Revealing the world of RNA interference. Mi, S. Miyoshi, T. Nakanishi, K. Structure of yeast Argonaute with guide RNA.

Parker, J. Persengiev, S. Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs siRNAs. Rna 10, 12— Pfaff, J. Argonaute and GW proteins: an effective alliance in gene silencing. Structural features of Argonaute-GW protein interactions.

U S A , E—E Pradhan, M. Plant Physiol. Rajewsky, N. Computational identification of microRNA targets. Schirle, N. Schlee, M. Discriminating self from non-self in nucleic acid sensing.

Schraivogel, D. Import routes and nuclear functions of Argonaute and other small RNA-silencing proteins. Trends Biochem. Seok, H. Cell Mol. Life Sci. Sharp, P. RNA interference. Sheridan, C. Sheu-Gruttadauria, J.

Sibisi, P. Plant Sci. Song, J. Song, X. Treiber, T. Regulation of microRNA biogenesis and its crosstalk with other cellular pathways. Wang, Y. Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes. Yan, K. Yi, R. Yuan, Y. Crystal Structure of A. Cell 19, — Zamore, P. Birmingham, A. Nat Protoc, Bramsen, J. Ui-Tei, K. Kittler, R. Nat Methods, Zhou, T. Dev Cell, Zender, L. PNAS, Hsieh, A. Nucleic Acids Res, Jiang, Z.

Kurisaki, K. Struwe, W. Methods Enzymol, Whitehurst, A. Bric, A. Cancer Cell, Kok, K. Lei, and D. Retrovirology,